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Team:Newcastle/9 August 2010
From 2010.igem.org
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| - | Today we will transform some competent ''E.coli'' DH5alpha with the ligation products: 1:5,1:3 and the vector | + | Today we will transform some competent ''E.coli'' DH5alpha with the ligation products: 1:5, 1:3 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]] |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:01, 9 August 2010
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Contents |
Transformation
Results/Aims
- pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Ligation
Aims
Today we will transform some competent E.coli DH5alpha with the ligation products: 1:5, 1:3 and the vector from the ligation on 06.08.10
   
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