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Team:Newcastle/14 July 2010
From 2010.igem.org
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
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| + | =LacI BioBrick Construction= | ||
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| + | ==Aims== | ||
| + | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | ||
Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests. | Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests. | ||
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Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday) | Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday) | ||
| - | Protocol | + | Protocol |
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| + | we followed Wendy's cards/Phil's lab-book | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 13:14, 6 August 2010
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LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests.
Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday)
Protocol
we followed Wendy's cards/Phil's lab-book
   
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