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Team:Newcastle/4 August 2010
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!'''Reverse Primer''' | !'''Reverse Primer''' | ||
!'''Melting Temperature (Tm in °C) ''' | !'''Melting Temperature (Tm in °C) ''' | ||
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!'''Size of the fragment''' | !'''Size of the fragment''' | ||
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|44.0 µl/ml | |44.0 µl/ml | ||
Revision as of 09:14, 5 August 2010
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Contents |
Cloning the rocF BioBrick
Aim
The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.
Materials and Protocol
Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:
PCR
| Tube | Part to be amplified | Plasmid consisting the part | Forward primer | Reverse Primer | Melting Temperature (Tm in °C) | Size of the fragment | Extension time* |
|---|---|---|---|---|---|---|---|
| 44.0 µl/ml | 19.9 µl/ml | 25.0 µl/ml | 30.8 µl/ml | 10.0 µl/ml | 44.2 µl/ml | 9.2 µl/ml | 39.7 µl/ml |
Gibson assembly
   
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