"
Team:TU Delft/4 August 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: =Alkane Sensing, Solvent Tolerance and Salt Tolerance= ''by Pieter'' We found it remarkable that the only positive samples we found so far were from the series without preamplifying the i...) |
(→Alkane Sensing, Solvent Tolerance and Salt Tolerance) |
||
| Line 3: | Line 3: | ||
We found it remarkable that the only positive samples we found so far were from the series without preamplifying the insert by PCR (e.g. the AlkS). This is why we decided to retry the insertion of bbc1, PhPFDalpha and PhPFDbeta in J61101, but this time without PCRing the insert. | We found it remarkable that the only positive samples we found so far were from the series without preamplifying the insert by PCR (e.g. the AlkS). This is why we decided to retry the insertion of bbc1, PhPFDalpha and PhPFDbeta in J61101, but this time without PCRing the insert. | ||
| + | |||
| + | Digestion | ||
| + | |||
| + | Ligation | ||
| + | |||
| + | Transformation | ||
=Emulsifier= | =Emulsifier= | ||
While waiting for the sequencing results of BBa_K398202 we already proceed with the insertion of OprG and AlnA. | While waiting for the sequencing results of BBa_K398202 we already proceed with the insertion of OprG and AlnA. | ||
Revision as of 12:42, 4 August 2010
Alkane Sensing, Solvent Tolerance and Salt Tolerance
by Pieter
We found it remarkable that the only positive samples we found so far were from the series without preamplifying the insert by PCR (e.g. the AlkS). This is why we decided to retry the insertion of bbc1, PhPFDalpha and PhPFDbeta in J61101, but this time without PCRing the insert.
Digestion
Ligation
Transformation
Emulsifier
While waiting for the sequencing results of BBa_K398202 we already proceed with the insertion of OprG and AlnA.
