Tuesday August 3, 2010

Jeremy

Today I did a restriction digest of the plasmids pSB1AK3 and pSB1AC3 for the plasmid switch of pRFP. This was also constructed together and transformed on AK and AC plates. Also today I designed a wiki main page template.

Raida

Today I set-up and ran a PCR with the forward primers that were designed for MalE and MalE31 with the signal sequence deleted. The PCR conditions are the same as before, with the annealing temperature being 54 degrees celcius for 30 seconds instead of 45 seconds.

I also did a restriction digest to check whether our insert (MalE, MalE31 C1, MalE31C2)had properly ligated to the TOPO vector.

At 4:00 pm, I set up and ran an Agarose Gel Electrophoresis of my PCR products and also of my restriction digest. For the PCR product, I am expecting a band around 1200 bp and for the restriction digest, one at 1400 and the other at 3500.

Today, I also worked on the Ethics paper. I have completed the Introduction to Synthetic Biology and Gene Expression Toolkit and have sent it to another team member to edit and give feedbacks.