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Team:Newcastle/4 August 2010
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==Aim== | ==Aim== | ||
| - | The aim of today's experiment is to construct the ''rocF'' BioBrick and clone it into [http://partsregistry.org/Part:pSB1C3 pSB1C3] using the [[Team:Newcastle/Gibson_Cloning|Gibson assembly method]]. | + | The aim of today's experiment is to construct the [[Team:Newcastle/Urease|''rocF'' BioBrick]] and clone it into [http://partsregistry.org/Part:pSB1C3 pSB1C3] using the [[Team:Newcastle/Gibson_Cloning|Gibson assembly method]]. |
==PCR== | ==PCR== | ||
Revision as of 20:26, 3 August 2010
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Contents |
Cloning the rocF BioBrick
Aim
The aim of today's experiment is to construct the rocF BioBrick and clone it into [http://partsregistry.org/Part:pSB1C3 pSB1C3] using the Gibson assembly method.
PCR
Gibson assembly
   
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