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Revision as of 16:30, 2 August 2010

Nina

IPTG induction on IgG protease-troubleshooting

I have had trouble overexpressing IgG protease (ideS), thus I ran two coomassie gels on IPTG treated BL21 samples. The gels were prepared according to our SDS-PAGE mixtures under protocol.

The IPTG treatment was carried out as once before. As a positive control I treated bFGF in its original vector with IPTG, since I knew it has been overexpressed previously. In addition I had each sample in two versions, such as one with and one without sonication. This was to investigate if the results would have any effect of the sonication. However, I don't recommend not sonicating samples since I observed they get very viscous when loading on to gels.

Arrangement on gels:

Andreas