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Team:Newcastle/14 June 2010
From 2010.igem.org
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==Tips== | ==Tips== | ||
| - | *Colony plates should be | + | *Colony plates should always be labeled at the base with the following information: |
| - | *For individual | + | #Name of the culture |
| + | #Initials of the person who made the plates | ||
| + | #Date | ||
| + | *For individual colony extraction, streak across a few times in different directions in order to dilute | ||
*Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame | *Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame | ||
| + | |||
==List of techniques that we did== | ==List of techniques that we did== | ||
Revision as of 12:59, 2 August 2010
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Aim
This is the first day of the iGEM lab training. In the beginning, we were given several important tips by Dr. Wendy Smith and were familiarized with the lab, the instruments present in the lab and finally were given a brief introduction on several instruments, lab based techniques and safety measures required to be maintained while working in lab.
Tips
- Colony plates should always be labeled at the base with the following information:
- Name of the culture
- Initials of the person who made the plates
- Date
- For individual colony extraction, streak across a few times in different directions in order to dilute
- Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame
List of techniques that we did
- Made broth culture
- Familiarise with using pipettes of different sizes
- Mini-Prep introduction for 15th June 2010.
- Used balance and made LB broth.
The biobrick BBa_J04450's prefix and suffix were identified. They are EcoRI and PstI respectively.
   
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