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Team:Newcastle/14 June 2010
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| - | This is the '''first''' | + | This is the '''first day''' of the iGEM lab training. At the beginning of the day, we were given several important tips by Dr. Wendy Smith and familiarized us with the lab, the instruments present in the lab and finally gave us a brief introduction on several instruments, lab based techniques and safety measures required to be maintained while working in lab. |
Revision as of 12:55, 2 August 2010
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Aim
This is the first day of the iGEM lab training. At the beginning of the day, we were given several important tips by Dr. Wendy Smith and familiarized us with the lab, the instruments present in the lab and finally gave us a brief introduction on several instruments, lab based techniques and safety measures required to be maintained while working in lab.
Tips
- Colony plates should be labelled (name of culture, our initial, date) at the base in order to prevent condensation.
- For individual clony extraction, streak across a few times in different directions in order to dilute
- Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame
List of techniques that we did
- Made broth culture
- Familiarise with using pipettes of different sizes
- Mini-Prep introduction for 15th June 2010.
- Used balance and made LB broth.
The biobrick BBa_J04450's prefix and suffix were identified. They are EcoRI and PstI respectively.
   
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