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Team:TU Delft/2 August 2010 content
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(New page: ==Alkane Sensing, Solvent Tolerance and Salt Tolerance== ''by Pieter'' I tried to assemble the remaining biobricks from various sub projects. The goal is to assemble the following: * AlkS...)
Newer edit →
(New page: ==Alkane Sensing, Solvent Tolerance and Salt Tolerance== ''by Pieter'' I tried to assemble the remaining biobricks from various sub projects. The goal is to assemble the following: * AlkS...)
Newer edit →
Revision as of 09:48, 2 August 2010
Alkane Sensing, Solvent Tolerance and Salt Tolerance
by Pieter
I tried to assemble the remaining biobricks from various sub projects. The goal is to assemble the following:
- AlkS + E0240
- PalkB + J06702
- J61101 + bbc1
- J61101 + PhPFDalpha
- J61101 + PhPFDbeta
To achieve this J06702, bbc1, PhPFDalpha and PhPFDbeta were amplified by PCR. The J61101 was cut open with S and P after which the biobricks were inserted. AlkS was inserted in the backbone with E0240.
