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Team:Newcastle/Growing an overnight cultures
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==Procedures== | ==Procedures== | ||
| - | # 5ml of LB broth is pipetted | + | # 5ml of LB broth is pipetted with the help of an auto pipette into a universal tube. |
| - | # The flame loop is used to pick a colony present on the agar plate. | + | # Heat the flame loop with the help of a burner till the loop is red hot to kill all the bacteria present on the loop. |
| + | # Let the flame loop to cool down for approximately 2 minutes. | ||
| + | # The flame loop is then used to pick a colony present on the agar plate. | ||
# The colony is then mixed into the LB Broth in the universal tube. | # The colony is then mixed into the LB Broth in the universal tube. | ||
| - | # The culture is left overnight at 37°C | + | # Again flame the flame loop till it gets red hot so as to kill any bacteria present on it. |
| + | # The culture is then left overnight at 37°C in an incubator. | ||
Revision as of 10:19, 30 July 2010
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Growing an Overnight Culture
Materials Required
- LB Broth
- Colonies of bacteria
- Flame loop
- Universal tube
- Pipette
Procedures
- 5ml of LB broth is pipetted with the help of an auto pipette into a universal tube.
- Heat the flame loop with the help of a burner till the loop is red hot to kill all the bacteria present on the loop.
- Let the flame loop to cool down for approximately 2 minutes.
- The flame loop is then used to pick a colony present on the agar plate.
- The colony is then mixed into the LB Broth in the universal tube.
- Again flame the flame loop till it gets red hot so as to kill any bacteria present on it.
- The culture is then left overnight at 37°C in an incubator.
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