Team:Harvard/color/notebook

From 2010.igem.org

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<span id="date"><a name="07-23-2010" class="date">07-23-2010</a></span> [<a class="top" href="#notebook">top</a>]<hr />
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<li>Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs</li>
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<li>PCR reaction on RS300 to insert recognition sites. Primers listed below</li>
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<li class="indent">LUT2 rxn 1: A, CP4</li>
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<li class="indent">LUT2 rxn 2: CP2, CP3</li>
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<li class="indent">LUT2 rxn 3: CP1, B</li>
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<li class="indent">BETA-OHASE 1 rxn 1: A, CP8</li>
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<li class="indent">BETA-OHASE 1 rxn 2: CP6, CP7</li>
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<li class="indent">BETA-OHASE 1 rxn 3: CP5, B</li>
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<li>PCR purified finished reaction and ran 4 ul on a 1% agarose gel</li>
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<div><a href="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png" id="single_image"><img src="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png"  width="100px"/> [click to enlarge]</a></div>
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<li>Cut out backbone (top) bands from O1, O2, R1 and saved them at 4&deg;C </li></ul>
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Revision as of 19:04, 28 July 2010



notebook

Week 6----07-23-2010
Week 707-26-201007-27-201007-28-201007-29-201007-30-2010


07-23-2010 [top]
  • Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs
  • PCR reaction on RS300 to insert recognition sites. Primers listed below
  • LUT2 rxn 1: A, CP4
  • LUT2 rxn 2: CP2, CP3
  • LUT2 rxn 3: CP1, B
  • BETA-OHASE 1 rxn 1: A, CP8
  • BETA-OHASE 1 rxn 2: CP6, CP7
  • BETA-OHASE 1 rxn 3: CP5, B
  • PCR purified finished reaction and ran 4 ul on a 1% agarose gel
  • Cut out backbone (top) bands from O1, O2, R1 and saved them at 4°C

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