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Team:Newcastle/23 June 2010
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The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone. | The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone. | ||
| - | ==== | + | ====Materials==== |
* ''E.coli'' broth culture | * ''E.coli'' broth culture | ||
* Pipettes | * Pipettes | ||
* Plasmid ligation products from yesterday | * Plasmid ligation products from yesterday | ||
| - | * Conical flasks | + | * Conical flasks |
====Transformation==== | ====Transformation==== | ||
Revision as of 14:24, 27 July 2010
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Contents |
Wednesday Morning
Aims
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
Materials
- E.coli broth culture
- Pipettes
- Plasmid ligation products from yesterday
- Conical flasks
Transformation
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1μl uncut
Wednesday afternoon - taking a break at the Hoppings!!!
Aims
The aim of this Lab practice was to have lots of fun!
Materials
- Candy floss
- Suntan lotion
- Teddy bears
- Burgers and bacon
- Chips and gravy
- Icecream
- Meerkat toys
- Madmouse ride!
- Beer, wine, lemonade
- Football
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