"
Team:Newcastle/26 July 2010
From 2010.igem.org
(Difference between revisions)
Swoodhouse (Talk | contribs) |
Swoodhouse (Talk | contribs) (→Preparation for cloning of the rocF BioBrick) |
||
| Line 2: | Line 2: | ||
==Preparation for cloning of the ''rocF'' BioBrick== | ==Preparation for cloning of the ''rocF'' BioBrick== | ||
| - | + | * Re-hydrated [http://partsregistry.org/Part:pSB1C3 pSB1C3] (the plasmid we will be submitting our BioBricks to the registry in) and [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] (double terminator we will be using for the ''rocF'' BioBrick) from the parts distribution. | |
==Colony PCR of Genomic DNA== | ==Colony PCR of Genomic DNA== | ||
Revision as of 19:37, 26 July 2010
| |||||||||||||
| |||||||||||||
Contents |
Preparation for cloning of the rocF BioBrick
- Re-hydrated [http://partsregistry.org/Part:pSB1C3 pSB1C3] (the plasmid we will be submitting our BioBricks to the registry in) and [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] (double terminator we will be using for the rocF BioBrick) from the parts distribution.
Colony PCR of Genomic DNA
Aim:
To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.
Materials:
- Pipette
- Microfuge
- Microtubes
- Distilled H2O
- Nucleotide DNTP
- 5x GoTaq buffer
- Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
- Forward and reverse primers
Protocol:
- For the full protocol, please refer to Colony PCR in Protocol List.
Conditions in ThermoCycler:
- Melting temperature, Tm used for Anneal step is 59°C.
Results:
Gel electrophoresis will be run tomorrow to determine the results.
Conclusion:
Please refer to Lab book dated 27.7.2010.
   
|
    
|
