"
Team:Newcastle/solution
From 2010.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
* Glue | * Glue | ||
* Directed movement | * Directed movement | ||
| + | |||
| + | === Types of Bacillus cells === | ||
| + | * Motile rod cells | ||
| + | * Filamentous | ||
| + | * Chain of rods | ||
| + | * Spores | ||
| + | * Lysed | ||
| + | * Biofilm | ||
| + | * Dividing cell | ||
| + | |||
| + | === Environment === | ||
| + | * Fermenter (Batch) | ||
| + | * External Face of Concrete | ||
| + | * Inside Crack | ||
| + | * Spray | ||
| + | * End of crack | ||
| + | * Non-target | ||
| + | |||
| + | === Experiments === | ||
| + | # Movement into cracks | ||
| + | # Speed of swimming in a particular direction | ||
| + | |||
| + | === Models === | ||
| + | # Rate of migration down micro crack | ||
| + | # Rate of protein production | ||
| + | # Mechanical model of repair strength | ||
| + | # Whole process. Signal propagation to release glue | ||
| + | |||
| + | === Concrete Questions === | ||
| + | * How big are micro cracks? | ||
| + | * How porous is concrete? | ||
| + | * How fast will concrete soak up liquid? | ||
| + | * Will a surfactant speed up this process? | ||
| + | * How to inspect for micro cracks: | ||
| + | *# fluorescence | ||
| + | *# ultra sound | ||
| + | |||
| + | === Parameters === | ||
| + | # Speed of swimming in a particular direction (wt) | ||
| + | # Quantities of glue, s.s, CaCO3, cell density | ||
| + | # Strength (tensile) of bonding agents, filamentous cells | ||
| + | |||
| + | === ??? === | ||
| + | # Grown in fermenter, rich medium | ||
| + | #* Rods | ||
| + | # Sporulation medium | ||
| + | #* spores | ||
| + | # Spray canister | ||
| + | #* spores | ||
| + | # Sprayed on concrete surface | ||
| + | #* germinate -> rods | ||
| + | # In crack | ||
| + | #* rods | ||
| + | # End of crack | ||
| + | #* Produce glue/CaCO3/silk/filaments | ||
| + | #* Produce signal peptides | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 14:47, 11 May 2010
| |||||||||||||
| |||||||||||||
Contents |
Required BioBricks
- 1) Sequential Programming (Richard)
- 2) End of crack & signaling system
- 3) Non-target-environment kill switch inc. inducible lysis
- 4) Spider silk & filamentous cells
- 5) Glue & lysis
- 6) CaCO3/Urease
Research teams
BioBrick 2 (End of crack & signaling system): Z.B.; P.H.; Y.E.; H.S.
BioBrick 3 (Non-target-environment kill switch): S.W.; A.L.; R.B.; J.S.; D.Y.
BioBrick 4 (Glue & lysis): J.S.; R.B.; S.W.; Y.E.; Z.B.
BioBrick 5 (CaCO3/Urease): H.S.; A.L.; P.H.; D.Y.
Characteristics / Actions
- Alkaline resistant
- Motility
- Sense Oxygen (for cracks)
- Quorum Sensing
- UV Sensitivity
- Produce glue/Silk
- Produce product in response to signal
Products
- Spider silk
- Glue
- Amyloid Fiber (forms Beta-Sheet)
- Calcium Carbonate
- Quorum Sensing Peptides
- Surfactin
Priorities
- Filamentous cells
- CaCO3
- Quorum Sensing
- Spider silk
- Glue
- Directed movement
Types of Bacillus cells
- Motile rod cells
- Filamentous
- Chain of rods
- Spores
- Lysed
- Biofilm
- Dividing cell
Environment
- Fermenter (Batch)
- External Face of Concrete
- Inside Crack
- Spray
- End of crack
- Non-target
Experiments
- Movement into cracks
- Speed of swimming in a particular direction
Models
- Rate of migration down micro crack
- Rate of protein production
- Mechanical model of repair strength
- Whole process. Signal propagation to release glue
Concrete Questions
- How big are micro cracks?
- How porous is concrete?
- How fast will concrete soak up liquid?
- Will a surfactant speed up this process?
- How to inspect for micro cracks:
- fluorescence
- ultra sound
Parameters
- Speed of swimming in a particular direction (wt)
- Quantities of glue, s.s, CaCO3, cell density
- Strength (tensile) of bonding agents, filamentous cells
???
- Grown in fermenter, rich medium
- Rods
- Sporulation medium
- spores
- Spray canister
- spores
- Sprayed on concrete surface
- germinate -> rods
- In crack
- rods
- End of crack
- Produce glue/CaCO3/silk/filaments
- Produce signal peptides
   
|
    
|
