Friday July 23, 2010

Gel electrophoresis of Raida's PCR products: Mal-E Gene specific primers

Chris's Gradient PCR gel electrophoresis product. The bands were more specific and showed around 400 bp, which is the size of the desired plasmid. A regular PCR and restriction digest will be run to continue to verify.

Raida

The first thing I did this morning was run a Gel Electrophoresis of my Mal-E Gene Specific Primer Testing PCR products. I ran it on a 1% gel at 100 V for an hour. Please refer to the image to the side. As it can be seen, the PCR results are positive. All the bands are near the 1200 bp band size, which indicates that it is the Mal-E gene. Furthermore, Lane 7 and Lane 13 show no band because this was the DNA that had the deleted sequence. So the fact that there is no band there is an indication of the fact that the MalE primer is functional and only amplified the MalE gene as it was supposed to. Further testing will be done to assure that the band shows the MalE Gene.

Chris

Today, I ran an agarose gel electrophoresis of the gradient PCR of CpxP promoter that was run overnight. The gel is shown on the right. The bands that showed up were mainly in the range of 400 bp which was the expected size. To confirm this size, a regular PCR was made and will run over the weekend as well as a restriction digest of the plasmids. The plasmids were then run on a 1% agarose gel electrophoresis and the gel is also shown to the right. As well, I continued contacting companies about sponsorship funding by phone and a package we put together. Next week, I plan to mail out our sponsorship packages to companies that require hard copies, hopefully sequence the CpxP promoter, and begin constructing it to RFP and GFP.