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Team:Calgary/23 July 2010
From 2010.igem.org
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[[Image:07.23.2010. MalEGeneSpec.jpg|thumb|400px|Gel electrophoresis of Raida's PCR products: Mal-E Gene specific primers]] | [[Image:07.23.2010. MalEGeneSpec.jpg|thumb|400px|Gel electrophoresis of Raida's PCR products: Mal-E Gene specific primers]] | ||
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| + | [[Image:07.23.2010. CpxP Gradient PCR.jpg|thumb|400px|Chris's Gradient PCR gel electrophoresis product. The bands were more specific and showed around 400 bp, which is the size of the desired plasmid. A regular PCR and restriction digest will be run to continue to verify.]] | ||
<u> Raida </u> | <u> Raida </u> | ||
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The first thing I did this morning was run a Gel Electrophoresis of my Mal-E Gene Specific Primer Testing PCR products. I ran it on a 1% gel at 100 V for an hour. Please refer to the image to the side. As it can be seen, the PCR results are positive. All the bands are near the 1200 bp band size, which indicates that it is the Mal-E gene. Furthermore, Lane 7 and Lane 13 show no band because this was the DNA that had the deleted sequence. So the fact that there is no band there is an indication of the fact that the '''MalE primer is functional and only amplified the MalE gene as it was supposed to'''. Further testing will be done to assure that the band shows the MalE Gene. | The first thing I did this morning was run a Gel Electrophoresis of my Mal-E Gene Specific Primer Testing PCR products. I ran it on a 1% gel at 100 V for an hour. Please refer to the image to the side. As it can be seen, the PCR results are positive. All the bands are near the 1200 bp band size, which indicates that it is the Mal-E gene. Furthermore, Lane 7 and Lane 13 show no band because this was the DNA that had the deleted sequence. So the fact that there is no band there is an indication of the fact that the '''MalE primer is functional and only amplified the MalE gene as it was supposed to'''. Further testing will be done to assure that the band shows the MalE Gene. | ||
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Revision as of 20:27, 23 July 2010
Friday July 23, 2010
Gel electrophoresis of Raida's PCR products: Mal-E Gene specific primers
Chris's Gradient PCR gel electrophoresis product. The bands were more specific and showed around 400 bp, which is the size of the desired plasmid. A regular PCR and restriction digest will be run to continue to verify.
Raida
The first thing I did this morning was run a Gel Electrophoresis of my Mal-E Gene Specific Primer Testing PCR products. I ran it on a 1% gel at 100 V for an hour. Please refer to the image to the side. As it can be seen, the PCR results are positive. All the bands are near the 1200 bp band size, which indicates that it is the Mal-E gene. Furthermore, Lane 7 and Lane 13 show no band because this was the DNA that had the deleted sequence. So the fact that there is no band there is an indication of the fact that the MalE primer is functional and only amplified the MalE gene as it was supposed to. Further testing will be done to assure that the band shows the MalE Gene.
No notebook page exists for this date. Sorry!
