Team:Cambridge/References/ProjectBioluminescence/Luciferase

(Difference between revisions)

Revision as of 10:12, 23 July 2010

[http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence]
[http://www.ncbi.nlm.nih.gov/pubmed/10529195 Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color]
[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis]

Vibrio Fischeri
Vibrio Harveyi
Genetics of luxCDABE (adapted from wikipedia)
[http://parts.mit.edu/wiki/index.php/Lux_operon| 2006 attempts at BioBricking the lux operon]
Notes
[http://partsregistry.org/Lux The lux wiki from parts registry]
[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"

[http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence]
[http://partsregistry.org/wiki/index.php/Part:BBa_I712052 Parts Registry Luciferase under control of Phage promoter] - T7 Phage
IMPORTANT INFO

[http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid experiment] -possible source of lux operon

[http://departments.kings.edu/biology/lux/index.html] - Transformation protocol for students from basics - includes several plasmids

Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?

@@ Line 17: / Line 17: @@
 *[[Team:Cambridge/ProjectBioluminescence/Luciferase/Notes| Notes]]
 *[http://partsregistry.org/Lux The lux wiki from parts registry]
+*[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
+*[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
 *[http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence]
-*[http://partsregistry.org/Part:BBa_G10001 parts registry lux operon] -currently waiting on an email to see how useful/available this part is
+*[http://partsregistry.org/wiki/index.php/Part:BBa_I712052 Parts Registry Luciferase under control of Phage promoter] - T7 Phage
 *[[Team:Cambridge/ProjectBioluminescence/Luciferase/IMPORTANT INFO | IMPORTANT INFO]]