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Team:Cambridge/References/ProjectBioluminescence
From 2010.igem.org
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* Characterise the transfer function of PoPs + CHBT --> Light | * Characterise the transfer function of PoPs + CHBT --> Light | ||
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* The Luciferin Cycle: | * The Luciferin Cycle: | ||
[[Image:Cam-luci-cycle.jpg | 600px]] | [[Image:Cam-luci-cycle.jpg | 600px]] | ||
Revision as of 13:59, 22 July 2010
Bioluminescence
The Plan
- Characterise the transfer function of PoPs + CHBT --> Light
The Theory
- The Luciferin Cycle:
Increasing Light Emission
- [http://www.ncbi.nlm.nih.gov/pubmed Paper on isolation of H-LRE and G-LRE]
Previous attempts to improve light production of luciferase reaction Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration
- [http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides]
- [http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase]
- [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase]
Sequences
- [http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
- [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant Luciferase (P.pyralis) Sequence]
Bacterial stuff
[http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid]
