Team:Newcastle/solution

Put explanation of alkaline resistant Bacillus subtilis growth on the wiki, maybe repeat the experiment.

Initial Research

Research

Required BioBricks

1. CaCO₃/Urease
2. Filamentous Cells
3. End of crack & signalling system
4. Swarming
5. Non-target-environment kill switch
6. Glue
7. Spider silk

Models

1. Rate of migration down micro crack
2. Rate of protein production
3. Mechanical model of repair strength
4. Whole process. Signal propagation to release glue

Characteristics / Actions

• Alkaline resistant
• Motility
• Sense Oxygen (for cracks)
• Quorum Sensing
• UV Sensitivity
• Produce glue/Silk
• Produce product in response to signal

Types of Bacillus cells

• Motile rod cells
• Filamentous
• Chain of rods
• Spores
• Lysed
• Biofilm
• Dividing cell

Environment

• Fermenter (Batch)
• External Face of Concrete
• Inside Crack
• Spray
• End of crack
• Non-target

Experiments

1. Movement into cracks
2. Speed of swimming in a particular direction

Parameters

1. Speed of swimming in a particular direction (wt)
2. Quantities of glue, s.s, CaCO3, cell density
3. Strength (tensile) of bonding agents, filamentous cells

 ???

1. Grown in fermenter, rich medium
   • Rods
2. Sporulation medium
   • spores
3. Spray canister
   • spores
4. Sprayed on concrete surface
   • germinate -> rods
5. In crack
   • rods
6. End of crack
   • Produce glue/CaCO3/silk/filaments
   • Produce signal peptides