Monday July 19, 2010

Emily

• Today I aliquoted our BBK-CP-F and BBK-CP-R primers. I then set up and ran a PCR with Taq Polymerase in order to test that our BBK construction primers are working. I also played around with the plate reader with our Arabinose induced cultures of I0500-B0034-E0040 from the weekend. Unfortunately, we didn't see any significant difference in GFP output between our cultures and a control of LB broth. We will start overnight cultures to retry the Arabinose induction procedure tonight. I also started overnight cultures of a J13002-E0040 construct in order to serve as a positive control in our GFP plate reading which we will be trying again tomorrow. Finally, I started a plasmid switch of our four constructs of I0500-B0034 in order to get them into a psb1AK3 vector and I also strated overnight cultures of B0034 in the psb1A2 plasmid, in order to start a plasmid switch of that construct tomorrow.

Patrick

Today, I set up and reupdated the team blog and Twitter. As well, I looked into papers regarding plasmid copy number and some basic JavaScript/CSS code to start constructing portions of the wiki.

Himika

Today I looked into plasmid copy number information which was assigned by Paul, who is the supervisor of our modeling component. I helped Emily set up the arabinose induced cultures but the readings of arabinose induction of I0500-B0034-E0040 versus LB broth(control) were not significantly different. Therefore, I believe that the RBS (b0034) did not get inserted as a result of GFP is not produced and readings are so similar. Due to this, I constructed I0500 with B0034.This is different from the previous constructions because this time I used I0500 as the insert so that it is easier to differentiate between the products that incorporated the insert versus products that did not. The products that incorporated the insert would be 1200 bp bigger than products that did not incorporate the insert. This is easier to screen.