Team:Stanford/NotebookPage/19 July 2010

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(Difference between revisions)
(New page: == Laura's Lab notebook == set up the following ligations from the gel extractions done by me, Karina, and Francisco on Friday, 7/14/10) *Francisco ran and imaged diagnostic gel on Friday ...)
(Laura's Lab notebook)
 
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*normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)
*normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)
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 +
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Nanodrop data (deemed unreliable based on 260/230, possibly due do residual EtBr contamination)
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{|
 +
|part || 260/280 || 260/230 || ng/uL
 +
|-
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|vector/terminator || 1.77 || 0.03 || 19.1
 +
|-
 +
|RFP || 1.86 || 0.02 || 16.2
 +
|-
 +
|GFP || 1.83 || 0.02 || 14.8
 +
|}

Latest revision as of 22:02, 19 July 2010

Laura's Lab notebook

set up the following ligations from the gel extractions done by me, Karina, and Francisco on Friday, 7/14/10)

  • Francisco ran and imaged diagnostic gel on Friday
Ligation Recipe
dH2O none
vector (with terminators attached) 5.0 uL
insert (RFP or GFP) 12.0 uL
10X buffer 2.0 uL
T4 ligase 1.0 uL
  • normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)


Nanodrop data (deemed unreliable based on 260/230, possibly due do residual EtBr contamination)

part 260/280 260/230 ng/uL
vector/terminator 1.77 0.03 19.1
RFP 1.86 0.02 16.2
GFP 1.83 0.02 14.8