Team:Minnesota

From 2010.igem.org

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<b>Metabolic Engineering: In vivo Nanobioreactors</b>
<b>Metabolic Engineering: In vivo Nanobioreactors</b>
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Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells.  Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates.  For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment.  Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes.  To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.  
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Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells.  Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates.  For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment.  Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes.  To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.
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<b>Athina Software Package</b>
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<b>A</b>utomated <b>Th</b>eoretical  is a web service to transform a sequence of BioBricks, or any other set of biomolecular components, to a set of reactions that can be simulated dynamically in SynBioSS DS. The user simply inputs the Biobricks and their relationships and Designer builds a reaction network using biological interactions rules. SynBioSS Wiki is a web service to collect the kinetic parameters necessary to create a model that can be simulated by SynBioSS designer.
<h2>Highlights for the Judges</h2>
<h2>Highlights for the Judges</h2>

Revision as of 20:35, 19 July 2010

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Welcome!

Welcome to the Team Minnesota Wiki for iGEM 2010!

We are a team of undergraduate and graduate students along with many advisors. This is the third year that Minnesota has sent a team to iGEM. Check back later for more updates!

The Mission

This year our team is pursuing two projects, one experimental and one computational. We are very excited about the potential of each of these projects. Check out our notebook and other pages for more details on these projects!

Metabolic Engineering: In vivo Nanobioreactors

Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment. Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes. To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.

Athina Software Package

Automated Theoretical is a web service to transform a sequence of BioBricks, or any other set of biomolecular components, to a set of reactions that can be simulated dynamically in SynBioSS DS. The user simply inputs the Biobricks and their relationships and Designer builds a reaction network using biological interactions rules. SynBioSS Wiki is a web service to collect the kinetic parameters necessary to create a model that can be simulated by SynBioSS designer.

Highlights for the Judges

Judges! Please navigate to our Judging Criteria page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.


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