Team:TU Delft/9 July 2010 content

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rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.

Revision as of 19:16, 19 July 2010

Lab work

Ordered DNA stocks

Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered DNA parts.

The following plasmid concentrations were obtained:

DNA part Concentration (ng/μL)
alkB2 147.3
ruBA3 63.6
rubA4 29.0
rubR 26.7
ladA 5011.2
ADH 178.8
ALDH 24.1
bbc1 5.5
AlnA 357.4
OprG 197.3
AlkS 510.8
PalkB 61.5
PalkS1-2 60.9
P(Caif) 9.7
PhPFDα 147.0
PhPFDβ 56.7

rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.