Team:TU Delft/9 July 2010 content
From 2010.igem.org
Lab work
Ordered DNA stocks
Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered DNA parts.
The following plasmid concentrations were obtained:
DNA part | Concentration (ng/μL) |
alkB2 | 147.3 |
ruBA3 | 63.6 |
rubA4 | 29.0 |
rubR | 26.7 |
ladA | 5011.2 |
ADH | 178.8 |
ALDH | 24.1 |
bbc1 | 5.5 |
AlnA | 357.4 |
OprG | 197.3 |
AlkS | 510.8 |
PalkB | 61.5 |
PalkS1-2 | 60.9 |
P(Caif) | 9.7 |
PhPFDα | 147.0 |
PhPFDβ | 56.7 |
rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.