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From 2010.igem.org

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Revision as of 19:15, 19 July 2010

LB media

   * 10 g LB broth
   * 500 mL DI water 

autoclave to sterilize

LB media with agar

   * 10 g LB broth
   * 7.5 g agar
   * 500 mL DI water 

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

Ampicillin Antibiotic Stock Solution (100 mg/mL)

   * 1 g of ampicillin
   * 5 mL of DI water
   * 5 mL of ethanol 

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

   * 250 mg of kanamycin
   * 5 mL of DI water 

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

   * 250 mg of tetracycline
   * 2.5 mL of DI water
   * 2.5 mL of ethanol 

filter sterilize and make 1 mL aliquots to store in the -20°C freezer