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Team:SDU-Denmark/labnotes2
From 2010.igem.org
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''Notes:'' To samples were run at two different temperatures: 56,1˚C and 64,5˚C respectively. <br>Polymerase used: Pfu<br> Primers used: None, as the two strands are supposed to anneal to each other<br><br> | ''Notes:'' To samples were run at two different temperatures: 56,1˚C and 64,5˚C respectively. <br>Polymerase used: Pfu<br> Primers used: None, as the two strands are supposed to anneal to each other<br><br> | ||
''Results:''<br><br> | ''Results:''<br><br> | ||
| + | === Gel extraction of flhD/C === | ||
| + | ''Start date:'' 19/07 ''End date:'' 19/07<br> | ||
| + | ''Methods:'' Gel extraction, nanodrop <br> | ||
| + | ''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.1 DE1.1] | ||
| + | ''Experiment done by:''Maria <br><br> | ||
| + | ''Notes:'' <br> | ||
| + | 70uL of flhD/C PCR product from [https://2010.igem.org/Team:SDU-Denmark/labnotes#Amplification_of_FlhDC_with_Pfu_and_mutation_of_stopcodon.2C_located_in_FlhD Amplification of flhD/C] was loaded onto a 1.5 agarose gel and extracted according to protocol. <br> | ||
| + | DNA was eluted in 20uL elution buffer.<br><br> | ||
| + | ''Results:''<br> | ||
| + | Nanodrop: | ||
| + | <table style="text-align: left; width: 100px;" border="1" | ||
| + | cellpadding="2" cellspacing="2"> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Sample ID<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">ng/uL<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">260/280<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">260/230<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">flhD/C 1<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">19.82<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">2.43<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">0.08<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">flhD/C 2<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">25.24<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">2.22<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">0.12<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br><br> | ||
| + | ''Analysis:''<br> | ||
| + | Nanodrop measurements idicated a possible contamination. However the DNA was pooled and used for digestion <br> | ||
| + | --[[User:Tipi|Tipi]] 17:22, 19 July 2010 (UTC) | ||
== Group: Photosensor == | == Group: Photosensor == | ||
Revision as of 17:22, 19 July 2010
