Team:TU Delft/19 July 2010 content

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|1 μg rubA4 + Xbal + PstI
|1 μg rubA4 + Xbal + PstI
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|Buffer
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|NEBuffer 2
|‘X – rubA4 – P’
|‘X – rubA4 – P’
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Revision as of 13:00, 19 July 2010

Lab work

Alkane degradation

Biobricks in production: Digestion reactions:

# Digestion reaction Used Buffer Needed fragment
1 2 μg J61100 + EcoRI + SpeI NEBuffer 2 ‘E – J61100 – S’
2 2 μg J61100 + EcoRI + SpeI NEBuffer 2 ‘E – J61100 – S’
3 1 μg rubR + EcoRI + SpeI NEBuffer 2 ‘E – rubR– S’
4 1 μg J61101 + EcoRI + SpeI NEBuffer 2 ‘E – J61101– S’
5 1 μg J61107 + EcoRI + SpeI NEBuffer 2 ‘E – J61107– S’
6 1μg alkB2 + Xbal + PstI NEBuffer 2 ‘X – alkB2 – P’
7 1 μg rubA3 + Xbal + PstI NEBuffer 2 ‘X – rubA3 – P’
8 1 μg rubA4 + Xbal + PstI NEBuffer 2 ‘X – rubA4 – P’
9 1 μg B0015 + Xbal + PstI NEBuffer 2 ‘X – B0015 – P’
10 1 μg ladA + Xbal + PstI NEBuffer 2 ‘X – ladA – P’
11 1 μg ADH + Xbal + PstI NEBuffer 2 ‘X – ADH – P’
12 1 μg ALDH + Xbal + PstI NEBuffer 2 ‘X – ALDH – P’
13 3 μg pSB1T3 + EcoRI + PstI NEBuffer 2 ‘X – ALDH – P’
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