Team:TU Delft/protocols/restriction enzyme digestion 40



in 40μl total volume:

Reaction for one sample:

DNA x μL (up to 1,0 μg)
Buffer 4,0 μL
Restriction enzymes 1 μL each (2x)
H2O x μL
BSA (100x) 0.4 μL
40 μL

Incubate for 2 hours at 37°C. Inactivate the restriction endonucleases by heat, incubation at 56 °C for 30 minutes and centrifuge shortly.