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Team:TU Delft/23 June 2010 content
From 2010.igem.org
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| - | + | ==Lab Work== | |
| - | [[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the digested BioBricks | + | <h4>Characterization of Anderson RBS sequences</h4> |
| + | [[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight digested BioBricks. The following ligation reactions was performed: | ||
| + | 1. I10341 + J1100 + pSB3C5 | ||
| + | 2. I10341 + J1101 + pSB3C5 | ||
| + | 3. I10341 + J1107 + pSB3C5 | ||
| + | 4. I10341 + J1117 + pSB3C5 | ||
| + | 5. I10341 + J1127 + pSB3C5 | ||
| + | 6. J1100 + pSB3C5 (negative control) | ||
| + | |||
| + | The general composition was: | ||
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| - | | | + | |'''Component''' |
| - | |Sample | + | |'''Sample''' |
|- | |- | ||
|I10341 | |I10341 | ||
| Line 24: | Line 33: | ||
|15 μL | |15 μL | ||
|} | |} | ||
| - | |||
| - | |||
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | ||
| - | + | The mixtures were incubated overnight at 16 °C. | |
Revision as of 18:05, 17 July 2010
Lab Work
Characterization of Anderson RBS sequences
Ligations were performed using the overnight digested BioBricks. The following ligation reactions was performed: 1. I10341 + J1100 + pSB3C5 2. I10341 + J1101 + pSB3C5 3. I10341 + J1107 + pSB3C5 4. I10341 + J1117 + pSB3C5 5. I10341 + J1127 + pSB3C5 6. J1100 + pSB3C5 (negative control)
The general composition was:
| Component | Sample |
| I10341 | 3 μL |
| J611XX | 4 μL |
| pSB3C5 | 1.5 μL |
| Ligation Buffer | 3 μL |
| T4 ligase | 1 μL |
| H20 | 15 μL |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The mixtures were incubated overnight at 16 °C.
