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Team:Calgary/15 July 2010
From 2010.igem.org
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*I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp. | *I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp. | ||
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| + | <u>Jeremy</u> | ||
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| + | *Today I reconstructed K239000 with I13507 and I13504, then K135000 with I13507 and I13504 with proper plating. I also did a restriction enzyme digest of pRFP with EcoRI and HindIII and also XbaI and HindIII. And a gel was run of the restriction enzyme products. Also 4 tubes were prepared for I0500+B0034+E0040 to test inducing arabinose. | ||
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Revision as of 23:19, 15 July 2010
Thurday July 15, 2010
Raida
- Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.
PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius
- I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
Jeremy
- Today I reconstructed K239000 with I13507 and I13504, then K135000 with I13507 and I13504 with proper plating. I also did a restriction enzyme digest of pRFP with EcoRI and HindIII and also XbaI and HindIII. And a gel was run of the restriction enzyme products. Also 4 tubes were prepared for I0500+B0034+E0040 to test inducing arabinose.
