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Team:Calgary/15 July 2010
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'''Thurday July 15, 2010''' | '''Thurday July 15, 2010''' | ||
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*Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with. | *Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with. | ||
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*I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp. | *I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp. | ||
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Revision as of 23:13, 15 July 2010
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Thurday July 15, 2010
Raida
- Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.
PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius
- I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
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