Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Transformation of MG1655 e. coli with BBa_274210, BBa_E0040 and BBa_I0500)
(Genomic DNA purification)
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''Methods:'' We used a ON culture of MG1655 cells <br><br>
''Methods:'' We used a ON culture of MG1655 cells <br><br>
''Protocols:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#GP1.1 GP1.1]<br><br>
''Protocols:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#GP1.1 GP1.1]<br><br>
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''Notes:'' We made four tupes with 300ul MG1655 cell media instead of 200ul. In tupe 1 and 2 I unfortunately added 800ul un-diluted precipitation solution, in tupe 3 and 4 I diluted the precipitation solution according to the GP1.1 protocol. The concentration of the purified DNA was measured by Nano Drop. Tybe 1 and 2 did not contain any DNA wich we had expected because of the high concentration of precipitation soultion. The DNA concentration i tube 3 and 4 are 28,38ng/uL and 19,4ng/uL, respectively. Tube 3 and 4 are efterwards pooled and saved in the freezer (labeled with white tag 1)
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''Notes:'' We made four tupes with 300ul MG1655 cell media instead of 200ul. In tupe 1 and 2 I unfortunately added 800ul un-diluted precipitation solution, in tupe 3 and 4 I diluted the precipitation solution according to the GP1.1 protocol. <br><br>
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<br><br>
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''Results:'' The concentration of the purified DNA was measured by Nano Drop. Tybe 1 and 2 did not contain any DNA wich we had expected because of the high concentration of precipitation soultion. The DNA concentration i tube 3 and 4 are 28,38ng/uL and 19,4ng/uL, respectively. Tube 3 and 4 are efterwards pooled and saved in the freezer (labeled with white tag 1) The purified DNA was run on a agarose gel to check if it actual contains DNA.
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''Results:'' As expected there were no result form tupe 1 and 2<br><br>
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--[[User:Louch07|Louise]] 10:44, 14 July 2010 (UTC)
--[[User:Louch07|Louise]] 10:44, 14 July 2010 (UTC)
--[[User:Pernm07|Pernm07]] 09:33, 14 July 2010 (UTC)
--[[User:Pernm07|Pernm07]] 09:33, 14 July 2010 (UTC)

Revision as of 09:57, 14 July 2010