Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Extraction of BBa_B0034 from pSB1A2 plasmid using PFU)
(Digestion of pSB1A2 w. B0034, pSB1AK3 w. B0015 and pSB3K3 w. J04450 and pSB1A2 using pstI(miniprep from 12/07))
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=== Digestion of pSB1A2 w. B0034, pSB1AK3 w. B0015 and pSB3K3 w. J04450 and pSB1A2 using pstI(miniprep from 12/07) ===
=== Digestion of pSB1A2 w. B0034, pSB1AK3 w. B0015 and pSB3K3 w. J04450 and pSB1A2 using pstI(miniprep from 12/07) ===
Start date: 13/07    End date: 13/07<br>
Start date: 13/07    End date: 13/07<br>
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''Methods:'' Digestion  <br><br>
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''Methods:'' Digestion  <br>
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''Protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<br><br>
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''Protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<br>
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''Notes:''Digestion of the plasmids with a single restriction enzyme was done to ensure that the plasmids extracted from mini-prep has the correct size. Digestion was done with half the amount of restriction mixture <br><br>
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''Experiment done by:'' Maria and Sheila<br><br>
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''Notes:''Digestion of the plasmids with a single restriction enzyme was done to ensure that the plasmids extracted from [https://2010.igem.org/Team:SDU-Denmark/labnotes#Mini-prep_of_pSB1A2_w._B0034.2C_pSB1AK3_w._B0015_and_pSB3K3_w._J04450.28transformation_from_08.2F07.29 mini-prep] has the correct size. Digestion was done with half the amount of restriction mixture <br><br>
samples were loaded into a 1.5% gel with a gene ruler red marker  <br><br>
samples were loaded into a 1.5% gel with a gene ruler red marker  <br><br>
''Results:''<br>
''Results:''<br>
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All bands had the size we expected for the plasmids. We therefore expect that we have isolated the right plasmids <br>
All bands had the size we expected for the plasmids. We therefore expect that we have isolated the right plasmids <br>
The samples from miniprep 12/7 was pooled and stored as no. 24, 25, 26 and 27 at -20 degrees C <br><br>
The samples from miniprep 12/7 was pooled and stored as no. 24, 25, 26 and 27 at -20 degrees C <br><br>
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--[[User:Tipi|Tipi]] 09:37, 14 July 2010 (UTC)
=== Transformation of MG1655 e. coli with BBa_274210, BBa_E0040 and BBa_I0500 ===
=== Transformation of MG1655 e. coli with BBa_274210, BBa_E0040 and BBa_I0500 ===

Revision as of 09:37, 14 July 2010