Team:KAIST-Korea/Notebook/Diary/July

July 1

We discussed whether our memo in wiki contains only the knowledge for other people or be literally just a memo for us. So we decided the memo to be literally the memo for our project. And we prof-read our introduction, motivation, memo, dairy together.

July 2

We faced new problem. Our main sponsor Bioneer president has no time because of business trip, we have no way to do the experiment for 10 days. So we discussed what to do during these 10 days. Since we have many advisors we need economical support. Therefore we decided to meet our department president for our economical support. And We will ask what we have to buy to KIB assistant for experiment. Finally we will ask Bioneer to give us a gene that we need right away, instead of doing it 10 days later.

Do list

1. Idea
   • what Bioneer use. (vector)
2. contacting professor Dong Sup for economical support.
3. Bioneer contact
4. experiment materials preparement.
5. Designing wiki.

July 5

It seems that gene synthesis will be slow when we look at bioneer information. Therefore we decided to buy JAK1 and STAT1 and do our experiment first. And since JAK1 is only sold outside of country we will test on JAK3 which has similar pathway instead. In case of fusion antibody, we think it would be slower for us to do the experiment we decided to let this task left for bioneer. And we will ask for advice how to do vector design. And we finished our diary design.

July 6

We asked for advice how to do vector design. So we give gene sequence and following restriction enzyme information to our advisor. So we got precious tips. And we estimated our budgets for our experiment.

July 7

1. Contacting Bioneer(for buying primer)
2. Buying genes from KRIBB and getting receipt.
3. Contacting Prof. Jung-Kyoon Choi for buying experimental equipments.
4. Buying JAK1 Gene, Antigen, Antibody.
5. Organize our budgets.
6. Contacting Prof. Seung-Uk Ryu for setting experimental equipments.

July 10

We changed our template to be yeast from e.coli. As a consiquence, we use IL-6 alpha receptor and changed into JAK1, gp130, LMO4, STAT3 included pathway. Also we researched on these gene sequence and give a modification to the introduction page.

July 12

We meet president of Bioneer for project advice. And we decided to change our target as tuberculosis.

Do list

1. Checking pathway (newly found that we have to put npKC-delta)
2. We discussed that we should change the menu because now we know how to get rid of main frame.
3. Giving a name for our project
4. finding new antibody sequence

July 13

1. background (yeast template, delete content about cancer cell)
2. design
3. motivation

July 14

We went to Bioneer to get advice on how to use yeast and design experiment. And they give us a information that we have to use homologous recombinant and it will take about 3 month. Therefore, we discussed what to do.

1. taking the risk of stability and put plasmid inside yeast.
2. we attend iGEM next year.

Finally we decided to conclude this problem when we have a final schedule for experiment.