Team:SDU-Denmark/labnotes

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(Lab notes (7/12 - 7/18))
(Polyferation of FlhDC with pfx)
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''Date'': july 13th <br><br>
''Date'': july 13th <br><br>
''methods'': The experiment was perform in the E.coli strain, MG1655. The DNA purification was done in duplicates and are saved in the freezer in tube 8 and 9. (green label). There DNA concentration was measure by Nano Drop and there contantretion are 16,87ng/uL and 19,25ng/uL, respectively. <br><br>
''methods'': The experiment was perform in the E.coli strain, MG1655. The DNA purification was done in duplicates and are saved in the freezer in tube 8 and 9. (green label). There DNA concentration was measure by Nano Drop and there contantretion are 16,87ng/uL and 19,25ng/uL, respectively. <br><br>
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''protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#Genomic_DNA_purification Genomic DNA purification] and [https://2010.igem.org/Team:SDU-Denmark/protocols#Colony_PCR colony PCR]. <br><br>
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''protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#Genomic_DNA_purification GP1.1] and [https://2010.igem.org/Team:SDU-Denmark/protocols#Colony_PCR CP1.1]. <br><br>
''Notes'': The experiment was generally preformed corresponding to the protocol but small modification was made. In the DNA purification experiment we used 300uL instead of 200uL of the overnight culture and it is important to remember that the 800uL precipitation sulution, containing 80uL solution and 720uL deionized water, are made directly before use. <br>
''Notes'': The experiment was generally preformed corresponding to the protocol but small modification was made. In the DNA purification experiment we used 300uL instead of 200uL of the overnight culture and it is important to remember that the 800uL precipitation sulution, containing 80uL solution and 720uL deionized water, are made directly before use. <br>
because our PRC for the time being havent been successful we are trying to do PCR with pfx wich are another polymerase with proofreading ability. The PRC program used for running PRC with pfx are as described below: <br><br>  
because our PRC for the time being havent been successful we are trying to do PCR with pfx wich are another polymerase with proofreading ability. The PRC program used for running PRC with pfx are as described below: <br><br>  

Revision as of 05:35, 14 July 2010