Team:Heidelberg/Project/Summary
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==miTuner== | ==miTuner== | ||
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- | We have developed a novel method for miRNA based gene expression tuning in mammalian cells. We show that the miTUNER method allows tuning of protein expression in fine intervals in vivo and in vitro. This enables us to create libraries of protein expression vectors with different strength without exchanging the promoter. This is highly useful if a certain promoter must be used, e.g. because it is optimal for a desired selective protein expression. | + | We have developed a novel-method for miRNA based gene expression tuning in mammalian cells. We show that the miTUNER method allows tuning of protein expression in fine intervals in vivo and in vitro. This enables us to create libraries of protein expression vectors with different strength without exchanging the promoter. This is highly useful if a certain promoter must be used, e.g. because it is optimal for a desired selective protein expression. |
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We furthermore demonstrate the ability of the miTuner approach for off-targeting respectively for partial, selective down-regulation of protein expression in vivo and in vitro. The potential of miTuner for on-targeting respectively partial, selective up-regulation has been proven in vitro, in vivo measurements are currently undertaken and will be presented at the Jamboree. To our best knowledge, this work is the first demonstration of a miRNA-based gene expression tuner, and it is moreover the first implementation of on-targeting with miRNA-based gene expression control systems.<br /> | We furthermore demonstrate the ability of the miTuner approach for off-targeting respectively for partial, selective down-regulation of protein expression in vivo and in vitro. The potential of miTuner for on-targeting respectively partial, selective up-regulation has been proven in vitro, in vivo measurements are currently undertaken and will be presented at the Jamboree. To our best knowledge, this work is the first demonstration of a miRNA-based gene expression tuner, and it is moreover the first implementation of on-targeting with miRNA-based gene expression control systems.<br /> |
Revision as of 03:54, 28 October 2010
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