Team:Heidelberg/Notebook/BSDesign/October
From 2010.igem.org
(Difference between revisions)
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*send for sequencing | *send for sequencing | ||
{{:Team:Heidelberg/Single_Bottom}} | {{:Team:Heidelberg/Single_Bottom}} | ||
+ | |||
+ | ---- | ||
+ | |||
+ | = 14/10/2010 = | ||
+ | * a reasonable | ||
+ | * Screening of 40 colonies of miR4286 (colony PCR) | ||
+ | * Screening of 32 colonies of miR320b (colony PCR) | ||
+ | |||
+ | |||
+ | all negative | ||
+ | <br /><br /><br /> | ||
+ | |||
+ | ---- | ||
+ | |||
+ | = 15/10/2010 = | ||
+ | |||
+ | ligation of miMeasure construct:<br /><br /> | ||
+ | |||
+ | miR-122<br /> | ||
+ | miR-122ran<br /> | ||
+ | miR-1179<br /> | ||
+ | miR-221<br /> | ||
+ | <br /> | ||
+ | each in 0S/0L/10S/10L versions | ||
+ | |||
+ | <br /><br /> | ||
+ | |||
+ | ---- | ||
+ | |||
+ | = 16/10/2010 = | ||
+ | transformation of each ligation to TOP10 ''E.coli'' | ||
+ | |||
+ | cells were plated on LB-amp plates | ||
+ | <br /><br /><br /> | ||
+ | |||
+ | ---- | ||
+ | |||
+ | = 17/10/2010 = | ||
+ | |||
+ | 96 colony PCRs - 8 from each plate | ||
+ | <br> | ||
+ | ALL NEGATIVE!!!! | ||
+ | <br /><br /><br /> | ||
+ | |||
+ | ---- |
Revision as of 03:54, 28 October 2010
![](https://static.igem.org/mediawiki/2010/9/92/Bg_plasmid.png)
Binding Site Design - October02/10/2010
05/10/2010
06/10/201020 Miniprep:
12/10/20104 miraPCRs for binding sites of miR4286.
13/10/2010
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