Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Transformation of MG1655 e. coli with BBa_274210, BBa_E0040 and BBa_I0500)
(Transformation of MG1655 e. coli with BBa_274210, BBa_E0040 and BBa_I0500)
Line 209: Line 209:
''Notes:''  
''Notes:''  
I0500 is located in pSB2K3. E0040 is in pSB1A2. 200ul cells were added to each tube. Positive control is RFP generator as usual. Negative control is just cells.<br><br>
I0500 is located in pSB2K3. E0040 is in pSB1A2. 200ul cells were added to each tube. Positive control is RFP generator as usual. Negative control is just cells.<br><br>
 +
Only 1ul DNA material was transferred per tube, since it was taken from distribution plates.<br><br>
Plates were dried at 42°C for 15 minutes, just prior to plating. Upon plating we discovered a problem with many of the ampicilin plates we made a couple of days ago. The agar breaks on the slightest contact, and are therfore impossible to use.<br><br>
Plates were dried at 42°C for 15 minutes, just prior to plating. Upon plating we discovered a problem with many of the ampicilin plates we made a couple of days ago. The agar breaks on the slightest contact, and are therfore impossible to use.<br><br>
As a result of the defective agar plates, most of our cells carrying BBa_E0040 were ruined. We managed to save and plate out two batches, one from each tube, so we are hoping for the best tomorrow.
As a result of the defective agar plates, most of our cells carrying BBa_E0040 were ruined. We managed to save and plate out two batches, one from each tube, so we are hoping for the best tomorrow.

Revision as of 18:35, 13 July 2010