Team:Heidelberg/Project/Capsid Shuffling/Homology Based
From 2010.igem.org
(→Results) |
Philippbayer (Talk | contribs) (→Results) |
||
Line 12: | Line 12: | ||
'''Selection in Huh-7 cells:''' | '''Selection in Huh-7 cells:''' | ||
- | Three selection rounds were carried out on Huh-7 cells, the viral DNA was isolated for each selection round and cloned into a Wilson AAV8 wild-type vector. 12 clones from each selection round were picked, and viruses that contain a YFP construct were produced and used to infect 96-well plates of mouse primary hepatocytes, HeLa and Huh-7 cells. | + | Three selection rounds were carried out on Huh-7 cells, the viral DNA was isolated for each selection round and cloned into a Wilson AAV8 wild-type vector. 12 clones from each selection round were picked, and viruses that contain a YFP construct were produced and used to infect 96-well plates of mouse primary hepatocytes, HeLa and Huh-7 cells. We already have some results for shuffled and selected AAV but they dont represent the whole variety of clones that are still breading inside the inczbator and will be tested with facs analysis this week. |
+ | |||
+ | [[Image:huh_clones.gif|350px]] | ||
+ | [[Image:hela_clones.gif|350px]] | ||
+ | [[Image:PH_clones.gif|350px]] | ||
'''Selection in Mouse Primary Hepatocytes:''' | '''Selection in Mouse Primary Hepatocytes:''' |
Revision as of 02:53, 28 October 2010
|
|
||