Team:Newcastle/8 September 2010

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{{Team:Newcastle/mainbanner}}
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=Sending off ''yneA'' BioBrick=
 
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==Aim==
 
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The aim of this experiment is to extract ligated ''yneA'' fragment in the plasmid pSB1C3 from ''E. coli'' cells and purify them and freeze dry them to send them off to the iGEM headquarters.
 
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==Materials and Protocol==
 
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Please refer to: [[Team:Newcastle/Qiagen Minipreps|Qiagen Minipreps]] for plasmid extraction from ''E. coli'' cells.
 
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Please refer to: [[TeamNewcastleNanoDrop Spectrophotometer| Nanodrop Spectrophotometer]] to check the purity of the extracted and purified plasmid.
 
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==Result==
 
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{|border=1
 
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|-
 
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!'''Tube'''
 
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!'''Nanodrop Value (in ng/ml)'''
 
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|-
 
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|1
 
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|158.7
 
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|-
 
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|2
 
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|261.2
 
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|-
 
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|3
 
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|262.9
 
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|-
 
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|4
 
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|204.7
 
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|-
 
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|5
 
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|262.9
 
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|-
 
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|6
 
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|202.1
 
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|-
 
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|7
 
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|174.8
 
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|-
 
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|8
 
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|226.6
 
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|-
 
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|9
 
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|229.1
 
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|-
 
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|10
 
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|164.5
 
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|-
 
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|11
 
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|177.8
 
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|-
 
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|12
 
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|225.1
 
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|-
 
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|13
 
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|281.5
 
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|-
 
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|14
 
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|249.3
 
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|-
 
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|15
 
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|192.2
 
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|16
 
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|239.3
 
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|}
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 02:16, 28 October 2010

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