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Team:Alberta/Notebook/TransformingCells
From 2010.igem.org
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The TSS transformation was tested. The procedure is listed here. | The TSS transformation was tested. The procedure is listed here. | ||
| - | One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same | + | [http://www.pnas.org/content/86/7/2172.abstract One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same |
| - | solution | + | solution] |
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*Acquisition of reagents- June 3, 2010 | *Acquisition of reagents- June 3, 2010 | ||
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*Cell preparation - June 7, 2010 | *Cell preparation - June 7, 2010 | ||
*Competency - June 10, 2010 | *Competency - June 10, 2010 | ||
| + | *Assessment of efficiency - | ||
==Rubidium chloride competent cells and lyophilization== | ==Rubidium chloride competent cells and lyophilization== | ||
Revision as of 02:06, 28 October 2010
Transforming Cells
Project Timeline: Click on an image to see more information
Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80oC freezers. We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.
TSS Transformation Protocol
The TSS transformation was tested. The procedure is listed here.
[http://www.pnas.org/content/86/7/2172.abstract One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same
solution]
- Acquisition of reagents- June 3, 2010
- Preparation of buffer - June 7, 2010
- Cell preparation - June 7, 2010
- Competency - June 10, 2010
- Assessment of efficiency -
Rubidium chloride competent cells and lyophilization
This method was adapted from a method used by Justin Fedor.
- Preparation of transformation buffer - July 6, 2010
- Preparation of cells - July 10, 2010
- Preparing competent cells - July 11, 2010
- Lyophilization - July 13, 2010
- Transformation of FD cells - July 14, 2010
- Preparation of cells 2 - July 30, 2010
- Lyophilization - August 2, 2010
- Transformation - August 5, 2010
