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Team:Queens-Canada/19 July 2010
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(New page: <h2>July 19, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Yuli Used 0.7 g agarose in 100 mL TBE. Gel run for 4...) |
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Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V. | Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | Practiced injections with water | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Revision as of 01:50, 28 October 2010
July 19, 2010
Gel Electrophoresis
Yuli
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Hao
pSra-10
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.
Liquid Culture
Hao
pSip-1::1A3 (Amp), BBa_I13522
Diagnostic Digestion
Yuli
pSip-1::1A3
Cut with: XbaI, SpeI, and PstI or NotI, EcoRI.
Gel Electrophoresis
Yuli and Chris
pSip-1 Digests
Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.
Microinjection
Hao
Practiced injections with water
