Team:TU Delft/30 June 2010 content
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We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010_content 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010_content 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | ||
+ | |||
+ | The following plasmid concentrations were obtained: | ||
+ | |||
+ | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
+ | |BioBrick | ||
+ | |Concentration (ng/μL | ||
+ | |- | ||
+ | |B0015 | ||
+ | |360.5 | ||
+ | |- | ||
+ | |B0032 | ||
+ | |80.4 | ||
+ | |- | ||
+ | |B0034 | ||
+ | |181.7 | ||
+ | |- | ||
+ | |E0040 | ||
+ | |66.0 | ||
+ | |- | ||
+ | |E0240 | ||
+ | |305.6 | ||
+ | |- | ||
+ | |E0422 | ||
+ | |411.0 | ||
+ | |- | ||
+ | |J61100 | ||
+ | |128.7 | ||
+ | |- | ||
+ | |J61101 | ||
+ | |167.7 | ||
+ | |- | ||
+ | |J61107 | ||
+ | |195.9 | ||
+ | |- | ||
+ | |J61117 | ||
+ | |62.7 | ||
+ | |- | ||
+ | |J61127 | ||
+ | |33.8 | ||
+ | |- | ||
+ | |R0010 | ||
+ | |40.0 | ||
+ | |- | ||
+ | |R0011 | ||
+ | |42.0 | ||
+ | |} | ||
+ | |||
''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010| Absorbance measurements] | ''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010| Absorbance measurements] |
Revision as of 11:57, 13 July 2010
Lab Work
We harvested the 50 mL bacterial cells of the 14 BioBricks. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation.
The following plasmid concentrations were obtained:
BioBrick | Concentration (ng/μL |
B0015 | 360.5 |
B0032 | 80.4 |
B0034 | 181.7 |
E0040 | 66.0 |
E0240 | 305.6 |
E0422 | 411.0 |
J61100 | 128.7 |
J61101 | 167.7 |
J61107 | 195.9 |
J61117 | 62.7 |
J61127 | 33.8 |
R0010 | 40.0 |
R0011 | 42.0 |
Pseudomonas Putida GPO1 is growing 1 day on different substrates. We measured absorbance of the Pseudomonas Putida to see on which conditions the strain survives. Absorbance measurements