Team:Newcastle/20 August 2010
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Revision as of 00:21, 28 October 2010
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Contents |
Transformation of ligated yneA into pGFPrrnB and pSB1C3
Aims
To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.
Materials and Protocol
Please refer to transformation of E. coli.
Plating Bacillus subtilis BFS678
Bacillus subtilis BFS678 has the pMutin4 (and thus lacI) integrated into the chromosome which will be useful for characterisation. Therefore we will be using this strain for transformation with our filamentous cell and arginase BioBricks.
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