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Team:Newcastle/18 June 2010
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| - | =Polymerase Chain | + | ==Polymerase Chain Reaction== |
| + | ===Protocol=== | ||
| - | Polymerase | + | Polymerase Chain Reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence. |
# To avoid contamination, wear gloves | # To avoid contamination, wear gloves | ||
# To achieve optimum results, always do everything on ice | # To achieve optimum results, always do everything on ice | ||
Revision as of 21:29, 12 July 2010
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Polymerase Chain Reaction
Protocol
Polymerase Chain Reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
- To avoid contamination, wear gloves
- To achieve optimum results, always do everything on ice
A basic PCR set up requires several components and reagents.These components include:
- DNA template that contains the DNA region (target) to be amplified.
- Two primers that are complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target.
- Taq polymerase or another DNA polymerase with a temperature optimum at around 70 °C.
- Deoxynucleoside triphosphates, the building blocks from which the DNA polymerases synthesizes a new DNA strand.
- Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
   
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