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Team:Newcastle/14 June 2010
From 2010.igem.org
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==Tips== | ==Tips== | ||
*Colony plates should be labelled (name of culture, our initial, date) at the base in order to prevent condensation. | *Colony plates should be labelled (name of culture, our initial, date) at the base in order to prevent condensation. | ||
| - | *For individual clony extraction, | + | *For individual clony extraction, streak across a few times in different directions in order to dilute |
| - | *Wear gloves, especially when working with DNA, PCR etc. However, never wear gloves when working near a flame | + | *Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame |
==List of techniques that we did== | ==List of techniques that we did== | ||
Revision as of 20:54, 12 July 2010
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This is the first day of the iGEM lab training. We were given severe important tips by Wendy to begin with, and then throughout the day, we had to familiarise with basic lab techniques, e.g. the use of pipettes.
Tips
- Colony plates should be labelled (name of culture, our initial, date) at the base in order to prevent condensation.
- For individual clony extraction, streak across a few times in different directions in order to dilute
- Wear gloves, especially when working with DNA, like PCR etc. However, never wear gloves when working near a flame
List of techniques that we did
- Made broth culture
- Familiarised with using pipettes of different sizes
- Mini-Prep introduction for 15th June 2010.
- Used balance and made LB broth.
The biobrick BBa_J04450's prefix and suffix were identified. They are EcoRI and PstI respectively.
   
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