Team:TU Delft/30 June 2010 content
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===Lab Work=== | ===Lab Work=== | ||
- | We harvested the 50 mL bacterial cells of the [ | + | We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010_content 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. |
''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010| Absorbance measurements] | ''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010| Absorbance measurements] |
Revision as of 19:05, 12 July 2010
Lab Work
We harvested the 50 mL bacterial cells of the 14 BioBricks. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation.
Pseudomonas Putida GPO1 is growing 1 day on different substrates. We measured absorbance of the Pseudomonas Putida to see on which conditions the strain survives. Absorbance measurements