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Team:METU Turkey/Results Discussion/Cell Imaging
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Cerenseref (Talk | contribs) (New page: <br>CLSM IMAGING <br>- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution. <br>- Spread the samples on the slide with the help of an inoculation loop....) |
Cerenseref (Talk | contribs) |
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<br>- Observe the slides under Brightfield 100X | <br>- Observe the slides under Brightfield 100X | ||
| - | <br>-Settings for GFP: | + | <br>- Settings for GFP: |
<br> HFT 488 | <br> HFT 488 | ||
<br> Ch 1 | <br> Ch 1 | ||
Latest revision as of 22:55, 27 October 2010
CLSM IMAGING
- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
- Spread the samples on the slide with the help of an inoculation loop.
- Wait for the sample to dry.
- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
- Observe the slides under Brightfield 100X
- Settings for GFP:
HFT 488
Ch 1
LP 505
Line 8
543 nm excitation
Laser power 5%
- Settings for RFP:
HFT 488/543
Ch 1
LP 560
Line 8
543 nm excitation
Laser power 10%
