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Team:Newcastle/Scanning EM
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This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications. | This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications. | ||
Revision as of 22:01, 27 October 2010
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Contents |
Scanning EM
This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications.
Fixation
Fix in 2% Gluteraldehyde in Sorensons Phosphate Buffer overnight (minimum). Rinse in Sorensons Phosphate Buffer 2x 15 mins.
Dehydration
25% ethanol: 30 mins. 50% ethanol: 30 mins. 75% ethanol: 30 mins. 100% ethanol: 1 hr. 100% ethanol: 1 hr.
Once in 100% ethanol, samples are dried using a Baltec Critical Point Dryer.
Mounting
Mount of an aluminium stub with Achesons Silver ElectroDag.
Gold coating
Coat with 15nm of gold using a Polaron SEM Coating Unit.
   
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